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Biology

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Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
 

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

Article DOI: 10.3791/50171-v 09:51 min March 5th, 2013
March 5th, 2013

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Summary

A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.

Tags

Keywords: Tetracycline-inducible Tet-on ShRNA CDNA Expression Gene Expression Mammalian Cell Lines RNA Interference Gene Knockdown Gene Overexpression Tet-off System Tet-on System TetR Tet-operator Cell Biology Gene Function Gene Manipulation
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