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JoVE Journal
Biology

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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
 

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Article doi: 10.3791/50180
March 30th, 2013 Usage Statistics

Summary March 30th, 2013

We present robust biochemical and microscopic methods for studying Caenorhabditis elegans lipid stores. A rapid, simple, fixing-staining procedure for fluorescent lipid droplet imaging leverages the spectral properties of the lipophilic dye Nile red. We then present biochemical measurement of triglycerides and phospholipids using solid phase extraction and gas chromatography-mass spectrometry.

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