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Giant Liposome Forberedelse til Imaging and Patch-Clamp Electrophysiology
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JoVE Journal Biology
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Giant Liposome Forberedelse til Imaging and Patch-Clamp Electrophysiology

DOI:
10.3791/50227-v

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09:03 min

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June 21, 2013

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1Department of Physiology and Biophysics,University of Washington

Chapters

  • 00:05Title
  • 01:19Deposition of Lipids from Organic Solvents: Classical Protocol
  • 03:13Electroformation of Giant Liposomes
  • 05:19Deposition of Lipids by Controlled Dehydration of Small Liposomes
  • 07:10Results: Electroformation of Giant Liposomes from Small Liposome Dispersions is Compatible with Reconstituted Ion Channel Function
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

Reconstituting funksjonelle membran proteiner i gigantiske liposomer definert sammensetning er en kraftig tilnærming når det kombineres med patch-clamp elektrofysiologi. Imidlertid kan konvensjonell gigantisk liposomen produksjonen være uforenlig med protein stabilitet. Vi beskriver protokoller for å produsere gigantiske liposomer fra rene lipider eller små liposomer som inneholder ionekanaler.

Tags

Giant LiposomesIon ChannelsElectrophysiologyPatch-clampElectroformationLipid MembranesReconstitutionSmall LiposomesDehydrationVapor EquilibriumSaturated Salt Solutions

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