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Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
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Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Article DOI: 10.3791/50248 • 08:36 min • April 12th, 2013
April 12th, 2013 •
1, 1,2
1Department of Craniofacial Biology, University of Colorado Denver Anschutz Medical Campus, 2Department of Cell and Developmental Biology, University of Colorado Denver Anschutz Medical Campus

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Summary
Automatic Translation

A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.

Tags

Mouse Embryonic Facial Ectoderm Mesenchyme Orofacial Clefts Craniofacial Defects Abnormal Facial Development Facial Prominences Frontal Nasal Processes Maxillary Prominences Mandibular Prominences Signaling Crosstalk Genes Involved Gene Expression Transcription Factor Binding Chromatin Marks Isolated Tissue Compartments Embryonic Day 10.5 Mouse Facial Formation
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