Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

DOI:

10.3791/50248-v

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08:36 min

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April 12, 2013

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Hong Li, Trevor Williams²

¹Department of Craniofacial Biology,University of Colorado Denver Anschutz Medical Campus, ²Department of Cell and Developmental Biology,University of Colorado Denver Anschutz Medical Campus

Chapters

00:05Title
01:34Dissecting CD-1 E10.5 Embryos
03:18Dispase II Treatment
04:13Separating the Facial Ectoderm and Mesenchyme
06:37Tissue Purity after Separation
07:26Conclusion

Summary

Automatic Translation

A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

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