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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Article DOI: 10.3791/50282
June 1st, 2014 •
1, 1
1Department of Neurology, Massachusetts General Hospital

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June 1st, 2014

We present a rapid and inexpensive screening method for identifying transcriptional regulators using high-throughput robotic transfections and a homemade dual-glow luciferase assay. This protocol rapidly generates direct side-by-side functional data for thousands of genes and is easily modifiable to target any gene of interest.

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