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JoVE Journal Biology
Generasjon av høy kvalitet Chromatin immunoprecipitation DNA Mal for High-throughput sekvensering (ChIP-seq)
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Generasjon av høy kvalitet Chromatin immunoprecipitation DNA Mal for High-throughput sekvensering (ChIP-seq)

Article DOI: 10.3791/50286
April 19th, 2013 •
1, 1, 1, 1,2
1Division of Human Genetics, Children's Hospital of Philadelphia Research Institute, 2Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania

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April 19th, 2013

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Kombinasjonen av kromatin immunoprecipitation og ultra-high-throughput sekvensering (ChIP-seq) kan identifisere og kartlegge protein-DNA vekselvirkninger i et gitt vev eller cellelinje. Skissert er hvordan å generere en høy kvalitet ChIP mal for etterfølgende sekvensering, ved hjelp av erfaring med transkripsjonsfaktor TCF7L2 som et eksempel.

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