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Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
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Biology
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JoVE Journal Biology
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

DOI:
10.3791/50307-v

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10:41 min

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July 24, 2014

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, , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics

Chapters

  • 00:05Title
  • 01:39Dissection Preparation
  • 03:15Presomitic Mesoderm Dissection and Dispersal
  • 06:40Imaging Dispersed Presomitic Mesodermal Cells
  • 07:58Image Processing of Acquired Time-lapse Movies
  • 08:58Representative Time-lapse Imaging Results
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.

Tags

Keywords: ZebrafishSegmentationPresomitic MesodermSomitesSegmentation ClockHer1Fluorescent ReporterIn Vitro CultureSingle-cell ImagingTime-lapse Imaging

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