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JoVE Journal
Immunology and Infection

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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
 

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Article doi: 10.3791/50323
June 23rd, 2013

Summary June 23rd, 2013

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

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