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En High-throughput Metode til måling af glomerulær filtrationshastighed i bevidst Mus
JoVE Journal
Biology
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JoVE Journal Biology
A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

En High-throughput Metode til måling af glomerulær filtrationshastighed i bevidst Mus

DOI:
10.3791/50330-v

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07:07 min

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May 10, 2013

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1Division of Nephrology-Hypertension, Department of Medicine,University of California, San Diego , 2San Diego VA Healthcare System

Chapters

  • 00:05Title
  • 01:39Preparation of FITC-inulin Injection Solution
  • 03:10Injection and Blood Collection
  • 05:23Preparation of Standards
  • 05:56Results: Ability of the FITC-inulin Single-bolus Injection Technique to Determine Changes in GFR
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

Måling af glomerulære filtrationshastighed (GFR) er den gyldne standard for nyrefunktionen vurdering. Her beskriver vi en high-throughput metode, der tillader bestemmelse af GFR i bevidste mus ved hjælp af en enkelt bolusinjektion, bestemmelse af fluoresceinisothiocyanat (FITC)-inulin i plasma og beregning af GFR ved en tofaset eksponentiel henfald model.

Tags

Glomerular Filtration RateGFRKidney Function AssessmentCreatinineInulinFluorescein IsothiocyanateFITC-inulinTwo-compartment ModelHigh-throughputConscious MiceNon-invasive

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