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En High-throughput Metode til måling af glomerulær filtrationshastighed i bevidst Mus
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En High-throughput Metode til måling af glomerulær filtrationshastighed i bevidst Mus

Article DOI: 10.3791/50330-v • 07:07 min • May 10th, 2013
May 10th, 2013 •
1,2
1Division of Nephrology-Hypertension, Department of Medicine, University of California, San Diego, 2San Diego VA Healthcare System

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Måling af glomerulære filtrationshastighed (GFR) er den gyldne standard for nyrefunktionen vurdering. Her beskriver vi en high-throughput metode, der tillader bestemmelse af GFR i bevidste mus ved hjælp af en enkelt bolusinjektion, bestemmelse af fluoresceinisothiocyanat (FITC)-inulin i plasma og beregning af GFR ved en tofaset eksponentiel henfald model.

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Medicin anatomi fysiologi Biomedical Engineering molekylærbiologi Nefrologisk nyrefunktionsundersøgelser glomerulær filtrationshastighed på rotter mus bevidst kreatinin inulin Jaffe hypertension HPLC dyremodel
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