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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
 

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Article doi: 10.3791/50354
September 10th, 2013

Summary September 10th, 2013

A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.

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