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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

DOI:
10.3791/50356-v

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10:47 min

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June 11, 2013

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, , , , , , ,
1Application Development,Akonni Biosystems, Inc., 2Manufacturing,Akonni Biosystems, Inc., 3Engineering,Akonni Biosystems, Inc., 4Research & Development,Akonni Biosystems, Inc.

Chapters

  • 00:05Title
  • 02:15The Hamilton STAR liquid-handling Robot
  • 02:51Setting Up a 96-well DNA Extraction from Whole Blood
  • 05:02Sample Lysis and Reagent Distribution
  • 07:31gDNA Extraction
  • 09:12Nucleic Acid Extraction, Speed, Fidelity and Purity
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.

Tags

Keywords: TruTip TechnologyNucleic Acid ExtractionAutomated Sample ProcessingClinical SamplesLiquid Handling RobotsRNA IsolationGenomic DNA IsolationFetal DNA ExtractionEppendorf EpMotion 5070Hamilton STARSTARplusHigh-throughputPorous Monolithic Binding MatrixCell LysisNucleic Acid BindingWashingElution

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