High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Rebecca C. Holmberg; Alissa Gindlesperger; Tinsley Stokes; Dane Brady; Nitu Thakore; Philip Belgrader; Christopher G. Cooney; Darrell P. Chandler

doi:10.3791/50356

/

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

34,424 Views

•

10:47 min

•

June 11, 2013

DOI:

10.3791/50356-v

DOI:

10.3791/50356-v

•

10:47 min

June 11, 2013

•

37 Views

Rebecca C. Holmberg¹, Alissa Gindlesperger¹, Tinsley Stokes¹, Dane Brady², Nitu Thakore³, Philip Belgrader³, Christopher G. Cooney³, Darrell P. Chandler⁴

¹Application Development,Akonni Biosystems, Inc., ²Manufacturing,Akonni Biosystems, Inc., ³Engineering,Akonni Biosystems, Inc., ⁴Research & Development,Akonni Biosystems, Inc.

Summary

TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.