Immunology and Infection
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Peptide-based Identification of Functional Motifs and their Binding Partners
Chapters
Summary June 30th, 2013
Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.
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