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JoVE Journal
Immunology and Infection

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Peptide-based Identification of Functional Motifs and their Binding Partners
 

Peptide-based Identification of Functional Motifs and their Binding Partners

Article DOI: 10.3791/50362-v 14:28 min June 30th, 2013
June 30th, 2013

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Summary

Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

Tags

Keywords: Peptide Motif Binding Partner HIV-1 Nef Apoptosis Secretion Modification Region (SMR) Exosome Mortalin Inhibitor
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