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JoVE Journal Medicine
Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
 

Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

Article doi: 10.3791/50375
August 14th, 2013 •
1,2,3,4, 1,2,3, 1,2,3, 1,2,3, 4, 1,2,3, 1,2,3
1U968, Institut National de la Santé et de la Recherche Médicale, 2UMR S 968, Université Pierre et Marie Curie, 3UMR 7210, Centre National de la Recherche Scientifique, 4Départment d'Ophtalmologie, Centre Hospitalier Universitaire de Bordeaux

Summary
Automatic Translation
August 14th, 2013

We used retinal samples from retinectomy for a transcriptomic analysis of retinal detachment. We developed a procedure that allows RNA conservation between the surgical blocks and the laboratory. We standardized a protocol to purify RNA by cesium chloride ultracentrifugation to assure that the purified RNAs are suitable for microarray analysis.

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