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Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
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JoVE Journal Medicine
Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

DOI:
10.3791/50375-v

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10:32 min

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August 14, 2013

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, , , , , ,
1U968,Institut National de la Santé et de la Recherche Médicale, 2UMR S 968,Université Pierre et Marie Curie, 3UMR 7210,Centre National de la Recherche Scientifique, 4Départment d’Ophtalmologie,Centre Hospitalier Universitaire de Bordeaux

Chapters

  • 00:05Title
  • 01:32Preparation of Sample Tubes for jouRNAl
  • 02:29Sample Collection
  • 03:28RNA Purification
  • 08:09Representative Analysis of Retinal Tissue-derived RNA
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

We used retinal samples from retinectomy for a transcriptomic analysis of retinal detachment. We developed a procedure that allows RNA conservation between the surgical blocks and the laboratory. We standardized a protocol to purify RNA by cesium chloride ultracentrifugation to assure that the purified RNAs are suitable for microarray analysis.

Tags

Keywords: Retinal DetachmentRetinal Pigmented EpitheliumPhotoreceptorVitrectomyRNA ExtractionTranscriptomicsGene Expression AnalysisInflammationPhotoreceptor Degeneration

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