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Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
JoVE Journal
Biology
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JoVE Journal Biology
Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
DOI:

12:41 min

May 31, 2013

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Chapters

  • 00:05Title
  • 01:43Dps Expression and Purification
  • 06:46DNA Protection Assay
  • 09:42Results: Purified Dps Protects DNA from Oxidative Degradation
  • 11:58Conclusion

Summary

Automatic Translation

The DNA-binding protein from starved cells (Dps) plays a crucial role in combating bacterial stress. This article discusses the purification of E. coli Dps and the protocol for an in vitro assay demonstrating Dps-mediated protection of DNA from degradation by reactive oxygen species.

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