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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

DOI:
10.3791/50391-v

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10:39 min

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July 05, 2013

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, , , , ,
1Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition,Children’s Hospital of Pittsburgh of UPMC, 2Department of Surgery,Tufts University Medical Center

Chapters

  • 00:05Title
  • 01:53Preparing Pancreatic Acinar Cells for Calcium Imaging
  • 04:09Measuring Calcium in Pancreatic Acinar Cells
  • 05:08Preparation of Pancreatic Acinar Cells for Cell Injury Assays
  • 06:30Measuring Lactate Dehydrogenase Leakage
  • 07:33Infecting Pancreatic Acinar Cells with Adenovirus
  • 08:01Results
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.

Tags

Keywords: Pancreatic Acinar CellsCalcium ImagingCell InjuryAdenoviral InfectionExocrine PancreasPancreatitisLactate DehydrogenaseCell IsolationCell TransfectionCell PhysiologyCell Pathology

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