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July 05, 2013
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We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.
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Cite this Article
Orabi, A. I., Muili, K. A., Wang, D., Jin, S., Perides, G., Husain, S. Z. Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection. J. Vis. Exp. (77), e50391, doi:10.3791/50391 (2013).
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