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Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples
 

Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples

Article DOI: 10.3791/50407
October 14th, 2013

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Summary October 14th, 2013

This protocol describes a high through put colorimetric method that relies on the formation of a complex between iodine and chains of glucose molecules in starch. Iodine forms complexes with both amylose and long chains within amylopectin. After the addition of iodine to a starch sample, the maximum absorption of amylose and amylopectin occurs at 620 and 550 nm, respectively. The amylose/amylopectin ratio can be estimated from the ratio of the 620 and 550 nm absorbance values and comparing them to a standard curve in which specific known concentrations are plotted against absorption values. This high throughput, inexpensive method is reliable and reproducible, allowing the evaluation of large populations of potato clones. 

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