Induction and Analysis of Epithelial to Mesenchymal Transition

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Induction and Analysis of Epithelial to Mesenchymal Transition

DOI:

10.3791/50478-v

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10:37 min

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August 27, 2013

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Yixin Tang, Greg Herr, Wade Johnson, Ernesto Resnik, Joy Aho

¹Antibody Development Department,R&D Systems, Inc., ²Stem Cells and Developmental Biology Department,R&D Systems, Inc.

Chapters

00:05Title
01:33Induction of EMT
03:09Analysis of Protein Expression by Immunocytochemistry
05:44Characterizing EMT-induced Human Cell Lines
09:47Conclusion

Summary

Automatic Translation

A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.

Induction and Analysis of Epithelial to Mesenchymal Transition

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