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A Sensitive Method to Quantify Senescent Cancer Cells
 

A Sensitive Method to Quantify Senescent Cancer Cells

Article DOI: 10.3791/50494-v 09:18 min August 2nd, 2013
August 2nd, 2013

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Summary

Whether senescence prevents or promotes tumorigenesis remains controversial. Since chemotherapeutical drugs can induce cancer cells to senesce, studying senescence is essential for proposing new therapies. However, the standard and broadly used β-galactosidase assay presents major drawbacks. We propose here a rapid and sensitive flow cytometry-based assay to quantify senescence.

Tags

Senescent Cancer Cells Quantification Method Cell Cycle Arrest Telomere Shortening Mitogenic Oncogenes Chemotherapeutic Drugs Tumorigenesis β-galactosidase Assay Flow Cytometry-based Assay Rapid Senescence Assessment Sensitive Senescence Detection Immunolabeling Of Cellular Markers
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