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Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
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Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

DOI:
10.3791/50548-v

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12:24 min

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August 31, 2013

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, , , , , ,
1School of Biochemistry,University of Bristol, 2Douglas Instruments

Chapters

  • 00:05Title
  • 01:58Preparation of the Seed Stock
  • 06:09Establishment of Crystallization Trays
  • 08:45Inspection of Crystallization Trays
  • 09:40Representative rMMS Experiment
  • 11:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a general method for random microseed matrix screening. This technique is shown to significantly increase the success rate of protein crystallization screening experiments, reduce the need for optimization, and provide a reliable supply of crystals for data collection and ligand-soaking experiments.

Tags

Keywords: Protein CrystallizationRandom Microseed Matrix ScreeningRMMSVapor DiffusionSitting DropHanging Drop96-well24-wellLiquid Handling RoboticsProtein Phase DiagramMetastable ZoneCrystal QualityCrystal SupplySoaking Experiments

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