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Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells

Article DOI: 10.3791/50568-v • 15:53 min • August 21st, 2013
August 21st, 2013 •
1, 2,3, 4, 4, 5, 1,6, 2,3
1Cancer Research Center, Sheba Medical Center, 2The Neufeld Cardiac Research Institute, Tel-Aviv University, 3Department of Physiology and Pharmacology, Tel-Aviv University, 4Imaging Unit, Sackler Faculty of Medicine, Tel-Aviv University, 5Biotechnology and Cell Signaling, Ecole Superieure de Biotechnologie Strasbourg, 6Department of Human Molecular Genetics and Biochemistry, Tel-Aviv University

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Summary
Automatic Translation

The cytotoxic activity of the phenanthridine PJ-34 in cancer cells undergoing mitosis was documented in real time by live confocal imaging. PJ-34 eradicated human breast cancer MDA-MB-231 cells harboring extra-centrosomes in mitosis. Unlike normal bi-focal mitosis, the extra-centrosomes were not clustered in the two spindle poles in the presence of PJ-34.

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Cell Death Abnormal Mitosis Confocal Imaging Live Cancer Cells PARP1 Inhibitors Phenanthrene Derivatives Supernumerary Centrosomes Multi-centrosomal Cells Solid Human Cancers PJ-34 Real-time Imaging Breast Cancer MDA-MB-231 Cells Fluorescent γ-tubulin Fluorescent Histone H2b Aberrant Chromosome Arrangements Declustered Centrosomes Spindle Poles Cytotoxic Activity
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