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Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

DOI:
10.3791/50599-v

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07:58 min

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June 19, 2013

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, , ,
1Department of Biology,Merrimack College, 2Center for Biotechnology and Biomedical Sciences,Merrimack College, 3Department of Technology R&D,Nexcelom Bioscience LLC

Chapters

  • 00:05Title
  • 01:39Infection of Macrophages with H. capsulatum, C. albicans
  • 02:21Macrophage Lysis
  • 02:56CFU Plating
  • 03:28Visualization of Fungi within Live Macrophages
  • 03:54Image Cytometric Analysis
  • 06:04Results: H. capsulatum in Macrophage Cells
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.

Tags

Keywords: Image CytometryPathogenic FungiCandida AlbicansHistoplasma CapsulatumCryptococcus NeoformansMacrophagesHost CellsColony Forming UnitFlow CytometryAcridine OrangePropidium IodideGFP-expressing Candida AlbicansQuantificationCellular PathogenesisInfection

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