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JoVE Journal
Bioengineering

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Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System
 

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Article DOI: 10.3791/50604
September 4th, 2013

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Summary September 4th, 2013

Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.

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