Journal
/
Medicine
/
A Protocol for Genetic Induction and Visualization of Benign and Invasive Tumors in Cephalic Complexes of Drosophila melanogaster
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
A Protocol for Genetic Induction and Visualization of Benign and Invasive Tumors in Cephalic Complexes of Drosophila melanogaster

A Protocol for Genetic Induction and Visualization of Benign and Invasive Tumors in Cephalic Complexes of Drosophila melanogaster

DOI:
10.3791/50624-v

•

07:23 min

•

September 11, 2013

•

1Department of Biology,Western Kentucky University

Chapters

  • 00:05Title
  • 01:24Induction of Invasive Tumors
  • 02:37Observation of Benign and Invasive Tumors
  • 04:13Dissection of the Cephalic Complex for Tumors
  • 06:16Benign and Invasive Tumor Imaging
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

Cooperation between an activated oncogene like RASV12 and mutations in cell polarity genes like scribbled, result in tumor growth in Drosophila where tumor cells also display invasive behaviors. Here a simple protocol for the induction and observation of the benign and invasive tumors is presented.

Tags

Drosophila MelanogasterGenetic InductionBenign TumorsInvasive TumorsCephalic ComplexesMARCM SystemRasV12Cell Polarity GenesTumor ProgressionTumor VisualizationTumor DissectionGenetic Basis Of Disease

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Accelerated Atherosclerosis in Mice: The “Wire-Injury” Model

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

Read Article