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Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
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Bioengineering
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JoVE Journal Bioengineering
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

English

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20,898 Views

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12:22 min

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July 22, 2013

DOI:

10.3791/50633-v

DOI:
10.3791/50633-v

•

12:22 min
July 22, 2013

•

2 Views
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1Department of Pathology and Cell Biology,Columbia University, 2Herbert Irving Comprehensive Cancer Center,Columbia University

Summary

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We describe the use of two ratiometric, genetically encoded biosensors, which are based on GFP, to monitor mitochondrial redox state and ATP levels at subcellular resolution in living yeast cells.

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Cite this Article

Vevea, J. D., Alessi Wolken, D. M., Swayne, T. C., White, A. B., Pon, L. A. Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells. J. Vis. Exp. (77), e50633, doi:10.3791/50633 (2013).

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