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Chapters
- 00:05Title
- 01:54Fabricating a Prototype cDEP Microfluidic Device
- 03:12Preparing a Cell Suspension in DEP Buffer
- 03:57Loading the cDEP Microfluidic Chip
- 06:04Characterizing Crossover Frequency of Cells Using cDEP Chip
- 07:49Varying the Experiment to Perform Cell Sorting or Enrichment
- 08:43Results: Cell Response and Sorting by Contactless Dielectrophoresis
- 09:50Conclusion
Contactless dielectrophoresis (cDEP) achieves sorting and enrichment of particles via their intrinsic dielectric properties. Fluidic electrode channels replace metallic electrodes traditional to DEP, suiting cDEP to non-damaging sterile characterization and sorting of biological particles. We demonstrate how to prepare a cDEP microdevice and conduct cell characterization and sorting experiments.
