Journal
/
Immunology and Infection
/
Concurrent Quantification of Cellular and Extracellular Components of Biofilms
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

DOI:
10.3791/50639-v

•

10:18 min

•

December 10, 2013

•

, , ,
1Department of Dental Materials, College of Dentistry,University of Oklahoma Health Sciences Center, 2Department of Biostatistics and Epidemiology, College of Public Health,University of Oklahoma Health Sciences Center, 3Department of Biological Sciences, School of Mathematics and Natural Sciences,The Copperbelt University

Chapters

  • 00:05Title
  • 01:45Specimen Fabrication
  • 02:23Biofilm Growth
  • 04:00Antibacterial/Mouthwash Treatments
  • 04:33Staining
  • 05:36Imaging Using Confocal Laser Scanning Microscopy
  • 06:55Biofilm Visualization and Structural Analysis
  • 07:56Results: Representative Biofilm Component Staining for Mouthwash Treatment and Control Groups
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.

Tags

BiofilmConfocal Laser Scanning MicroscopyExtracellular Polymeric SubstancesProteinsNucleic AcidsStreptococcus MutansResin CompositesMouthwashesBiovolumeMean Biofilm Thickness

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50639?article_type=v

Neutrophil Extracellular Traps: How to Generate and Visualize Them
check_url/50639?article_type=v

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging
check_url/50639?article_type=v

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
check_url/50639?article_type=v

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
check_url/50639?article_type=v

Growth of Mycobacterium tuberculosis Biofilms
check_url/50639?article_type=v

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
check_url/50639?article_type=v

Ratiometric Imaging of Extracellular pH in Dental Biofilms
check_url/50639?article_type=v

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
check_url/50639?article_type=v

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
check_url/50639?article_type=v

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Read Article