Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Sharukh S. Khajotia; Kristin H. Smart; Mpala Pilula; David M. Thompson

doi:10.3791/50639

Journal

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Immunology and Infection

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Concurrent Quantification of Cellular and Extracellular Components of Biofilms

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

8,248 Views

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10:18 min

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December 10, 2013

DOI:

10.3791/50639-v

DOI:

10.3791/50639-v

•

10:18 min

December 10, 2013

•

11 Views

Sharukh S. Khajotia¹, Kristin H. Smart¹, Mpala Pilula^1,3, David M. Thompson²

¹Department of Dental Materials, College of Dentistry,University of Oklahoma Health Sciences Center, ²Department of Biostatistics and Epidemiology, College of Public Health,University of Oklahoma Health Sciences Center, ³Department of Biological Sciences, School of Mathematics and Natural Sciences,The Copperbelt University

Summary

A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.