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Antibody Staining in C. Elegans Using "Freeze-Cracking"
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Antibody Staining in C. Elegans Using "Freeze-Cracking"

Antibody Staining in C. Elegans Using "Freeze-Cracking"

DOI:
10.3791/50664-v

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13:10 min

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October 14, 2013

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1Department of Biological Sciences,Ohio University

Chapters

  • 00:05Title
  • 01:13Preparation of Frozen Nematode Slides
  • 04:17Preparation of Slides of Individual Nematodes
  • 05:32Fixation: Light Fix of Methanol-Acetone
  • 06:30Fixation: Hard Fix Using Formaldehyde or Glutaraldehyde
  • 08:22Preparation of Slides for Microscopy
  • 10:32Representative Images
  • 12:45Conclusion

Summary

Automatic Translation

Automatic Translation

"Freeze-cracking," a method for exposing the inner tissues of the nematode C. elegans to antibodies for protein localization, is demonstrated.

Tags

Antibody StainingC. ElegansFreeze-crackingCuticleNematodesMechanical PermeabilizationFixativesSample RecoveryMorphologyOptimizationChemical Permeabilization

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