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JoVE Journal
Biology

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Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
 

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Article doi: 10.3791/50684
October 8th, 2013 Usage Statistics

Summary October 8th, 2013

Electroporation is a commonly employed method for introducing DNA into bacteria in a process known as transformation. Traditional protocols for the preparation of electrocompetent cells are time consuming and labor intensive. This article describes an alternate, rapid, and efficient method for the preparation of electrocompetent cells presently employed by some laboratories.

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