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Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
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Bioengineering
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JoVE Journal Bioengineering
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

DOI:
10.3791/50736-v

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09:01 min

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September 27, 2013

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, ,
1Department of Orthopaedic Surgery,Stanford University , 2Department of Bioengineering,Stanford University

Chapters

  • 00:05Title
  • 01:58Polymer Synthesis
  • 03:57Nanoparticle Preparation and Transfection
  • 04:55Cell Injections
  • 05:49Bioluminescence Imaging
  • 06:38Tissue Harvest Procedure
  • 07:07Results: Angiogenesis after Nanoparticle Transfection
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the method of programming stem cells to overexpress therapeutic factors for angiogenesis using biodegradable polymeric nanoparticles. Processes described include polymer synthesis, transfecting adipose-derived stem cells in vitro, and validating the efficacy of programmed stem cells to promote angiogenesis in a murine hindlimb ischemia model.

Tags

Stem CellsAngiogenesisTherapeutic AngiogenesisBiodegradable Polymeric NanoparticlesVascular GrowthIschemic DiseasesGene TherapyAdipose-derived Stem CellsVEGFHindlimb Ischemia Model

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