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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
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Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Article DOI: 10.3791/50736
September 27th, 2013 •
*1, *1,2, 1,2
1Department of Orthopaedic Surgery, Stanford University, 2Department of Bioengineering, Stanford University
* These authors contributed equally

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September 27th, 2013

We describe the method of programming stem cells to overexpress therapeutic factors for angiogenesis using biodegradable polymeric nanoparticles. Processes described include polymer synthesis, transfecting adipose-derived stem cells in vitro, and validating the efficacy of programmed stem cells to promote angiogenesis in a murine hindlimb ischemia model.

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