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Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

DOI:
10.3791/50750-v

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11:12 min

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November 01, 2013

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, , ,
1Sophie Davis School of Biomedical Education,The City College of New York, 2Cell & Developmental Biology,University of California, San Diego

Chapters

  • 00:05Title
  • 01:38Embryo Collection and Preparation
  • 04:33Embryo Wounding
  • 06:18Embryo Microinjection
  • 07:34Formaldehyde Fixation Notes
  • 09:00Gene Expression at Wound Sites
  • 10:45Conclusion

Summary

Automatic Translation

Automatic Translation

The embryonic epidermis of very late stage Drosophila embryos provides an in vivo system for rapid puncture wound response analysis and can be combined with genetic manipulations or chemical microinjection treatments to advance studies in wound healing for translation into mammalian models.

Tags

Keywords: DrosophilaEmbryoEpidermalWound ResponseMicroinjectionPuncture InjuryWound Transcriptional ReportersHemocyte RecruitmentWound HealingRegenerative ResponseInnate Immune Response

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