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Immunology and Infection

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JoVE Journal Immunology and Infection
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Article DOI: 10.3791/50779 • 09:03 min • September 5th, 2013
September 5th, 2013 •
1, 1,2,3,4,5, 1,2,3,4,5
1Centre for Antimicrobial Resistance, Alberta Health Services / Calgary Laboratory Services / University of Calgary, 2Department of Pathology & Laboratory Medicine, University of Calgary, 3Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, 4Department of Medicine, University of Calgary, 5The Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases, University of Calgary

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Summary
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We demonstrate a simple multiplex PCR assay for quick-screening and typing of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V for methicillin-resistant Staphylococcus aureus, and provide some of the vital steps and procedural nuances that make it successful for adapting this assay to individual laboratories.

Tags

Multiplex PCR Assay Staphylococcal Cassette Chromosome Mec (SCCmec) Typing Methicillin-resistant Staphylococcus Aureus (MRSA) Epidemiology Clonal Strain Relatedness Community-associated MRSA (CA-MRSA) PCR Typing Schemes Primer Sets Individual PCR Experiments Multiplex PCR Assay SCCmec Types Subtypes I To V Sequence Information Assay Adaptation Laboratories MRSA SCCmec Assay
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