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Immunology and Infection

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JoVE Journal Immunology and Infection
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Met Click Chemie aan het effect van de virale infectie Meet op Host-cel RNA Synthese

Article DOI: 10.3791/50809-v • 09:35 min • August 9th, 2013
August 9th, 2013 •
1, 1, 1, 1, 1
1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch

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Deze methode beschrijft het gebruik van klikchemie op veranderingen in gastheercel transcriptie meten na infectie met het Rift Valley fever virus (RVFV) stam MP-12. De resultaten kunnen worden gevisualiseerd kwalitatief via fluorescentie microscopie of verkregen kwantitatief via flowcytometrie. Deze methode kan worden aangepast voor gebruik met andere virussen.

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Immunologie Virologie Chemie Infectieziekten biochemie genetica moleculaire biologie Cellulaire Biologie Geneeskunde Biomedische Technologie Arbovirussen, RNA Nucleair transcriptie Genetic Rift Valley fever virus NSS transcriptie click chemie MP-12 fluorescentie microscopie flowcytometrie virussen proteïnen immunokleuring assay
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