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Chapters
- 00:05Title
- 02:44Yeast Luminometry Method
- 04:23cRNA Injection
- 06:08Two-electrode Voltage-clamp
- 08:11Patch Clamp Examination of Direct Mechanosensitivity
- 09:41Results: Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
- 11:38Conclusion
To complement commonly used methods to study TRPV4’s, two methods are described: Its mechanosensitivity can be studied by Ca2+-aequorin luminometry in transgenic yeast upon hypo-osmotic challenge. It can also be examined in TRPV4-RNA injected Xenopus oocytes by whole-cell two-electrode voltage clamp or patch clamp in on-cell or excised mode.
