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Gær luminometriske og<em> Xenopus</em> Oocyte elektrofysiologiske Undersøgelser af Molekylær Mechanosensitivity af TRPV4
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Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Gær luminometriske og<em> Xenopus</em> Oocyte elektrofysiologiske Undersøgelser af Molekylær Mechanosensitivity af TRPV4

DOI:
10.3791/50816-v

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12:09 min

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December 31, 2013

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, , ,
1Laboratory of Cell and Molecular Biology,University of Wisconsin – Madison, 2Department of Genetics,University of Wisconsin – Madison

Chapters

  • 00:05Title
  • 02:44Yeast Luminometry Method
  • 04:23cRNA Injection
  • 06:08Two-electrode Voltage-clamp
  • 08:11Patch Clamp Examination of Direct Mechanosensitivity
  • 09:41Results: Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
  • 11:38Conclusion

Summary

Automatic Translation

Automatic Translation

Som supplement til almindeligt anvendte metoder til at studere TRPV4 s er to metoder beskrevet: Dens mechanosensitivity kan studeres af Ca 2 +-aequorin-luminometri i transgene gær ved hypo-osmotisk udfordring. Det kan også undersøges i TRPV4-RNA injicerede Xenopus oocytter ved hel-celle to-elektrode spænding klemme eller patch clamp i on-celle eller fjernede tilstand.

Tags

Keywords: TRPV4MechanosensitivityYeast LuminometryXenopus Oocyte ElectrophysiologyCalcium SignalingPatch ClampTransgenic ExpressionHereditary Bone/neuronal Pathologies

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