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Gær luminometriske og
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Gær luminometriske og

Article DOI: 10.3791/50816-v • 12:09 min • December 31st, 2013
December 31st, 2013 •
1, 1, 1, 1,2
1Laboratory of Cell and Molecular Biology, University of Wisconsin – Madison, 2Department of Genetics, University of Wisconsin – Madison

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Som supplement til almindeligt anvendte metoder til at studere TRPV4 s er to metoder beskrevet: Dens mechanosensitivity kan studeres af Ca 2 +-aequorin-luminometri i transgene gær ved hypo-osmotisk udfordring. Det kan også undersøges i TRPV4-RNA injicerede Xenopus oocytter ved hel-celle to-elektrode spænding klemme eller patch clamp i on-celle eller fjernede tilstand.

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Basic-protokollen Eukaryota Archaea bakterier Life Sciences (General) Mechanosensation Ionkanaler lipider patch clamp, gær luminometri force sensing spænding klemme TRPV4 elektrofysiologi
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