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Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
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Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells

Article DOI: 10.3791/50819 • 07:34 min • January 10th, 2014
January 10th, 2014 •
1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine

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Summary
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The material here describes a method developed to preserve the three-dimensional chromatin structure of testicular germ cells. This has been termed the three-dimensional (3D) slide method. This method improves sensitivity for detection of subnuclear structures and is applicable for immunofluorescence, DNA, and RNA fluorescence in situ hybridization (FISH).

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Slide Preparation Method Three-dimensional Chromatin Architecture Testicular Germ Cells Testicular Tubules Permeabilization Step Fixation Step Cytospin Immunofluorescence DNA Fluorescence In Situ Hybridization (FISH) RNA Fluorescence In Situ Hybridization (FISH) Cot-1 RNA FISH Nascent RNAs Spatial Relationships Chromatin Structure
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