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JoVE Journal
Immunology and Infection

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In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
 

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

Article doi: 10.3791/50823
January 6th, 2014

Summary January 6th, 2014

Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.

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