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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Journal (Immunology and Infection)
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
Journal (Immunology and Infection)
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
 

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

Article DOI: 10.3791/50823
January 6th, 2014 •
1,2,3, 1,3, 3, 1,3
1Department of Pediatrics, Harvard Medical School, 2Department of Pediatric Gastroenterology, MGH for Children, 3Mucosal Immunology and Biology Research Center, Massachusetts General Hospital

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January 6th, 2014

Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.

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