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Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
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JoVE Journal Chemistry
Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

DOI:
10.3791/50839-v

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11:37 min

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November 29, 2013

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1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH),University of Heidelberg

Chapters

  • 00:05Title
  • 02:39Preparation for Amide Hydrogen-exchange and Hydrogen Exchange Mass Spectrometry (HX-MS)
  • 04:09Determination of Peptic Peptides Using MS/MS Spectra
  • 05:54Identification of Protein-protein Interaction Interfaces
  • 08:48Results: Analysis of Protein Dynamics
  • 11:04Conlusion

Summary

Automatic Translation

Automatic Translation

Protein conformation and dynamics are key to understanding the relationship between protein structure and function. Hydrogen exchange coupled with high-resolution mass spectrometry is a versatile method to study the conformational dynamics of proteins as well as characterizing protein-ligand and protein-protein interactions, including contact interfaces and allosteric effects.

Tags

Keywords: Hydrogen ExchangeMass SpectrometryProtein DynamicsProtein ConformationProtein FoldingProtein-protein InteractionsEnzyme CatalysisAllosteryStructural Analysis

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