Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Nikolai Hentze; Matthias P. Mayer

doi:10.3791/50839

Journal

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Chemistry

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Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

18,285 Views

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11:37 min

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November 29, 2013

DOI:

10.3791/50839-v

DOI:

10.3791/50839-v

•

11:37 min

November 29, 2013

•

17 Views

Nikolai Hentze¹, Matthias P. Mayer¹

¹Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH),University of Heidelberg

Summary

Protein conformation and dynamics are key to understanding the relationship between protein structure and function. Hydrogen exchange coupled with high-resolution mass spectrometry is a versatile method to study the conformational dynamics of proteins as well as characterizing protein-ligand and protein-protein interactions, including contact interfaces and allosteric effects.