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Immunology and Infection

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JoVE Journal Immunology and Infection
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)
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Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

Article DOI: 10.3791/50854 • 06:36 min • January 2nd, 2014
January 2nd, 2014 •
1
1Department of Biochemistry, Microbiology and Immunology, University of Ottawa

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Summary
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This protocol allows for a direct comparison between planktonic and biofilm resistance for a bacterial strain that can form a biofilm in vitro using a 96-well microtiter plate. Planktonic or biofilm bacteria are exposed to serial dilutions of the antimicrobial agent of choice. Viability is assayed by growth on agar plates.

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Minimal Bactericidal Concentration Antimicrobial Agent Planktonic Cells Biofilm Cells Comparison Bacterial Strain In Vitro 96-well Microtiter Plate Serial Dilutions Bacterial Suspensions Biofilm Formation Unattached Cells Media Replenishment Exposure To Antimicrobial Agent Growth Assay Biofilm Recovery Cell Viability Assay MBC-P MBC-B
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