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Bioengineering

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JoVE Journal Bioengineering
Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties
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Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

Article DOI: 10.3791/50857-v • 14:42 min • May 2nd, 2014
May 2nd, 2014 •
1, 1, 2, 3, 1,3
1Physicochime Curie, CNRS UMR 168, Institut Curie, Sorbonne Universités, UPMC, 2Unité de Génétique des Biofilms, Institut Pasteur, 3Laboratoire Jean Perrin, CNRS UMR 8237, Sorbonne Universités, UPMC

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This paper shows an original methodology based on the remote actuation of magnetic particles seeded in a bacterial biofilm and the development of dedicated magnetic tweezers to measure in situ the local mechanical properties of the complex living material built by micro-organisms at interfaces.

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Remote Magnetic Actuation Micrometric Probes In Situ 3D Mapping Bacterial Biofilm Physical Properties Adhesion And Growth Extracellular Polymeric Substances Local Physical Properties Macroscopic And Homogeneous Material Perspective Magnetic Particles Remote Actuation Structural Properties Magnetic Tweezers Microscope Recording Time-lapse Images Particle Trajectories Viscoelastic Parameters 3D-spatial Distribution
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