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Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties
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Bioengineering
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JoVE Journal Bioengineering
Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties

DOI:
10.3791/50857-v

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14:42 min

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May 02, 2014

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, , , ,
1Physicochime Curie, CNRS UMR 168, Institut Curie,Sorbonne Universités, UPMC, 2Unité de Génétique des Biofilms,Institut Pasteur, 3Laboratoire Jean Perrin, CNRS UMR 8237,Sorbonne Universités, UPMC

Chapters

  • 00:05Title
  • 01:28Magnetic Particle Preparation
  • 02:29Channel Preparation and Biofilm Growth
  • 07:28Magnetic Tweezers Installation
  • 09:27Creep Curve Acquisition
  • 11:02Analysis
  • 12:26Representative in situ Measurements of the Local Physical Properties of a Bacterial Biofilm
  • 14:18Conclusion

Summary

Automatic Translation

Automatic Translation

This paper shows an original methodology based on the remote actuation of magnetic particles seeded in a bacterial biofilm and the development of dedicated magnetic tweezers to measure in situ the local mechanical properties of the complex living material built by micro-organisms at interfaces.

Tags

BiofilmBacterial BiofilmExtracellular Polymeric SubstancesEPSMagnetic ParticlesMagnetic Tweezers3D MappingViscoelastic PropertiesIn Situ MeasurementBacterial AdhesionBiofilm Control

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