Journal
/
Medicine
/
Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

DOI:
10.3791/50868-v

•

11:02 min

•

October 30, 2013

•

, , , , , , ,
1Department of Internal Medicine, Division of Hematology and Oncology,University of California, Davis, 2Comparative Pathology Laboratory, UC Davis School of Veterinary Medicine,University of California, Davis, 3Merck Serono Research,Merck KGaA, Darmstadt, Germany

Chapters

  • 00:05Title
  • 01:43Bladder Cancer Induction and Vaccination Treatments
  • 02:49Monitoring and Sample Collection
  • 04:11Multiplex Fluorometric Microbead Immunoassay
  • 06:37IFN-γ/IL-4 ELISpot Preparation and Analysis
  • 08:07Representative Transitional Cell Bladder Carcinoma Induction Results
  • 10:39Conclusion

Summary

Automatic Translation

Automatic Translation

An N-butyl-N-(4-hydroxybutyl)nitrosamine-induced bladder cancer model was developed in human mucin 1 (MUC1) transgenic mice for the purpose of testing MUC1-directed immunotherapy. After administering a MUC1-targeted peptide vaccine, a cytotoxic T lymphocyte response to MUC1 was confirmed by measuring serum cytokine levels and T-cell specific activity.

Tags

Keywords: Bladder CancerTransitional Cell CarcinomaSquamous Cell CarcinomaMUC1 TransgenicN-butyl-N-(4-hydroxybutyl)nitrosamineCytokinesImmunotherapyPeptide VaccineT-cell Response

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50868?article_type=v

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
check_url/50868?article_type=v

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
check_url/50868?article_type=v

Protocol for Long Duration Whole Body Hyperthermia in Mice
check_url/50868?article_type=v

The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation
check_url/50868?article_type=v

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
check_url/50868?article_type=v

Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts
check_url/50868?article_type=v

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice
check_url/50868?article_type=v

Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury
check_url/50868?article_type=v

Induction of Accelerated Atherosclerosis in Mice: The “Wire-Injury” Model
check_url/50868?article_type=v

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

Read Article