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Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
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Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

DOI:
10.3791/50879-v

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09:38 min

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November 29, 2013

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, ,
1School of Molecular Biosciences,Washington State University, 2Center for Reproductive Biology,Washington State University

Chapters

  • 00:05Title
  • 01:23Preparation of Fatty Acid Supplemented Medium
  • 03:28Inducing Germ Cell Destruction by DGLA Supplementation
  • 04:51Confirming Fatty Acid Uptake by Gas Chromatography
  • 06:43Representative Fatty Acid Supplementation Results
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

Caenorhabditis elegans is a useful model to explore the functions of polyunsaturated fatty acids in development and physiology. This protocol describes an efficient method of supplementing the C. elegans diet with polyunsaturated fatty acids.

Tags

Keywords: Fatty AcidsCaenorhabditis ElegansPolyunsaturated Fatty AcidsOmega-3Omega-6Dietary SupplementationMembrane CompositionEnergy StorageSignaling PathwaysGenetic ToolsGas Chromatography

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