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Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

DOI:
10.3791/50919-v

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11:20 min

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November 07, 2013

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,
1Department of Pediatrics,Washington University School of Medicine, 2Department of Molecular Microbiology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:27Seeding and Growing 5637 Cells on Permeable Supports |
  • 03:46Isolation of Human Neutrophils from Peripheral Blood
  • 06:22Transuroepithelial Neutrophil Migration Assay
  • 09:56Results: PMN Migration Across Bladder Epithelia in Response to Various Stimuli
  • 10:59Conclusion

Summary

Automatic Translation

Automatic Translation

We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.

Tags

Keywords: Neutrophil MigrationBladder EpitheliumUrinary Tract InfectionUropathogenic Escherichia ColiInnate Immune ResponseBoyden ChamberTransuroepithelial Migration AssayChemoattractantEukaryotic CellsPMN Traversal

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