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JoVE Journal
Immunology and Infection

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Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
 

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Article DOI: 10.3791/50919
November 7th, 2013

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Summary November 7th, 2013

We developed an in vitro model that mimics an important component of the acute inflammatory response during infection of the bladder with uropathogenic Escherichia coli. The transuroepithelial neutrophil migration assay enables quantitative assessment of human neutrophil migration across bladder epithelia, cultured on permeable supports, in response to bacterial infection or chemoattractant substances.

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