24,824 Views
•
09:22 min
•
October 30, 2013
DOI:
Summary
Automatically generated
The preparation of high quality yeast cell extracts is a necessary first step in the analysis of individual proteins or entire proteomes. Here we describe a fast, efficient, and reliable homogenization protocol for budding yeast cells that has been optimized to preserve protein functions, interactions, and post-translational modifications.
Related Videos
12:41
Measuring Replicative Life Span in the Budding Yeast
Related Videos
20556 Views
07:47
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Related Videos
52960 Views
12:04
Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
Related Videos
13338 Views
15:02
Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
Related Videos
10692 Views
07:13
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
Related Videos
17243 Views
10:07
Identification of Post-translational Modifications of Plant Protein Complexes
Related Videos
23774 Views
14:43
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Related Videos
11492 Views
11:16
Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
Related Videos
36865 Views
12:28
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Related Videos
12016 Views
11:02
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
Related Videos
28722 Views
Read Article
Cite this Article
Szymanski, E. P., Kerscher, O. Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications. J. Vis. Exp. (80), e50921, doi:10.3791/50921 (2013).
Copy