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JoVE Journal
Biology

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Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
 

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Article DOI: 10.3791/50921
October 30th, 2013

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Summary October 30th, 2013

The preparation of high quality yeast cell extracts is a necessary first step in the analysis of individual proteins or entire proteomes. Here we describe a fast, efficient, and reliable homogenization protocol for budding yeast cells that has been optimized to preserve protein functions, interactions, and post-translational modifications.

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