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Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays
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Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

DOI:
10.3791/50925-v

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08:01 min

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December 13, 2013

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, ,
1Division of Genetics and Genomics, Manton Center for Orphan Disease Research,Boston Children’s Hospital, Harvard Medical School

Chapters

  • 00:05Title
  • 01:44In vivo Analysis of Skeletal Muscle Structure by Birefringence
  • 04:23Touch-evoked Escape Behavior Assay
  • 05:10Results: Representative WT and Mutant Skeletal Muscle Analyses
  • 07:28Conclusion

Summary

Automatic Translation

Automatic Translation

The zebrafish is now an established and powerful tool for modeling muscular dystrophies, congenital myopathies, and related neuromuscular diseases. Birefringence and touch-evoked escape behavior are two common noninvasive assays used to determine the degree of muscular disorganization and locomotive impairment of zebrafish embryos during early development.

Tags

Keywords: BirefringenceTouch-evoke Escape ResponseSkeletal Muscle DefectsZebrafishMuscular DystrophyCongenital MyopathySarcomeric AssemblyMyofiber DegenerationRegenerationMyopathicIn VivoVertebrate Model

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