Journal
/
Biology
/
Imaging Centrosomes in Fly Testes
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Imaging Centrosomes in Fly Testes

Imaging Centrosomes in Fly Testes

DOI:
10.3791/50938-v

•

09:41 min

•

September 20, 2013

•

, , ,
1Department of Biological Sciences,University of Toledo

Chapters

  • 00:05Title
  • 01:14Preparation of Live Testes
  • 02:26Preparation of Fixed Testes
  • 03:27Preparation of Immunostained Testes
  • 07:59Analyzing Chromosome Elongation
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

Imaging of centrosomal proteins during Drosophila spermatogenesis is a powerful method to identify new proteins critical for centrosome biology as well as to elucidate the particular function of known players in this process.

Tags

CentrosomesMicrotubulesCell CycleCell DifferentiationCell DivisionCiliaDrosophilaSpermatogenesisGenetic ModelMutantsLight MicroscopyGenetically-tagged MarkersGenetic Screens

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Imaging Cell Shape Change in Living Drosophila Embryos

Isolation of Drosophila melanogaster Testes

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Identification of Novel Regulators of Plant Transpiration by Large-Scale Thermal Imaging Screening in Helianthus Annuus

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

Read Article