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Chemistry

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Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
 

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Article DOI: 10.3791/50969
February 18th, 2014

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Summary February 18th, 2014

Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

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