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Chapters
- 00:05Title
- 01:52Synthesis of Lipid-stabilized Micro and Nanoparticles
- 03:21Determination of Particle Size
- 04:03Preparation of Mice for i.LN. Injection
- 05:31i.LN. Injection of Particles
- 06:33Results: Particle Synthesis, Animal Preparation, and Lymph Node Injection
- 07:19Results: Tracer Dye Drainage
- 07:38Results: Visualization of Particles in Injection Lymph Nodes
- 08:03Conclusion
Lymph nodes are the immunological tissues that orchestrate immune response and are a critical target for vaccines. Biomaterials have been employed to better target lymph nodes and to control delivery of antigens or adjuvants. This paper describes a technique combining these ideas to inject biocompatible polymer particles into lymph nodes.
