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JoVE Journal
Biology

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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
 

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Article DOI: 10.3791/50985
February 18th, 2014

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Summary February 18th, 2014

We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.

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