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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Article DOI: 10.3791/50985 • 16:43 min • February 18th, 2014
February 18th, 2014 •
1,2,3, 2,3,4, 2,3, 1,2
1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine, University of Milan, 3Neuroscience Institute, National Research Council (CNR), 4Department of Health Science, "Magna Graecia" University of Catanzaro

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Summary
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We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.

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Endoplasmic Reticulum Subdomains Cultured Cells Protein And Lipid Mobility Trafficking Live-cell Imaging Fluorescence Recovery After Photobleaching (FRAP) Fluorescence Loss In Photobleaching (FLIP) Intracellular Trafficking Exo-endocytic Pathway Organelles Subcompartments Protein Complexes Protein Localization Lipid Microdomains Physiological Environment Limitations Fluorescent Fusion Proteins Optical Microscopy Fine Structure Of The ER
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