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Visualisering af endoplasmatiske reticulum Subdomains i dyrkede celler
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JoVE Journal Biology
Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualisering af endoplasmatiske reticulum Subdomains i dyrkede celler

DOI:
10.3791/50985-v

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16:43 min

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February 18, 2014

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, , ,
1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine,University of Milan, 3Neuroscience Institute,National Research Council (CNR), 4Department of Health Science,“Magna Graecia” University of Catanzaro

Chapters

  • 00:05Title
  • 01:37Plasmid, Cell Culture, and Transfection with ER Fluorescent Proteins
  • 03:21Live Fluorescence Scanning Confocal Microscopy
  • 08:35Ultrastructural Analysis by Means of Transmission Electron Microscopy
  • 12:38Representative Imaging Results
  • 16:04Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver de billeddannende metoder, vi bruger til at undersøge fordelingen og mobilitet for de transficerede fluorescerende proteiner er hjemmehørende i det endoplasmatiske reticulum (ER) ved hjælp af konfokal billedbehandling af levende celler. Vi har også ultrastrukturelt analysere effekten af ​​deres udtryk på arkitektur i dette subcellulære rum.

Tags

Endoplasmic ReticulumCell CompartmentsProtein TraffickingLipid MobilityFRAPFLIPLive-cell ImagingFluorescence MicroscopyElectron MicroscopyOrganelle SubcompartmentsPhysiological ConditionsPathological Conditions

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